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MiR-486 <t>promotes</t> <t>AKT/mTOR</t> signalling activation under OGD/R conditions in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of <t>p-AKT/AKT</t> expression in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative western blot images and quantitative analysis of p-S6K/S6K expression in H9c2 cells under the indicated conditions. ( C ) Representative western blot images and quantitative analysis of p-4EBP1/4EBP1 expression in H9c2 cells under the indicated conditions. Compared with OGD/R or mimic NC groups, miR-486 mimic significantly increased phosphorylation levels of AKT, S6K, and 4EBP1, whereas the miR-486 inhibitor further reduced their phosphorylation levels. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001. NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
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MiR-486 <t>promotes</t> <t>AKT/mTOR</t> signalling activation under OGD/R conditions in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of <t>p-AKT/AKT</t> expression in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative western blot images and quantitative analysis of p-S6K/S6K expression in H9c2 cells under the indicated conditions. ( C ) Representative western blot images and quantitative analysis of p-4EBP1/4EBP1 expression in H9c2 cells under the indicated conditions. Compared with OGD/R or mimic NC groups, miR-486 mimic significantly increased phosphorylation levels of AKT, S6K, and 4EBP1, whereas the miR-486 inhibitor further reduced their phosphorylation levels. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001. NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
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MiR-486 <t>promotes</t> <t>AKT/mTOR</t> signalling activation under OGD/R conditions in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of <t>p-AKT/AKT</t> expression in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative western blot images and quantitative analysis of p-S6K/S6K expression in H9c2 cells under the indicated conditions. ( C ) Representative western blot images and quantitative analysis of p-4EBP1/4EBP1 expression in H9c2 cells under the indicated conditions. Compared with OGD/R or mimic NC groups, miR-486 mimic significantly increased phosphorylation levels of AKT, S6K, and 4EBP1, whereas the miR-486 inhibitor further reduced their phosphorylation levels. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001. NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion
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p akt ser473  (Proteintech)
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Antibodies used in this study
P Akt Ser473, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt/Phospho-AKT+(Ser473)+Antibody/pmc13637308-39-0-3
Average 96 stars, based on 1 article reviews
p akt ser473 - by Bioz Stars, 2026-11
96/100 stars
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Image Search Results


MiR-486 promotes AKT/mTOR signalling activation under OGD/R conditions in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of p-AKT/AKT expression in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative western blot images and quantitative analysis of p-S6K/S6K expression in H9c2 cells under the indicated conditions. ( C ) Representative western blot images and quantitative analysis of p-4EBP1/4EBP1 expression in H9c2 cells under the indicated conditions. Compared with OGD/R or mimic NC groups, miR-486 mimic significantly increased phosphorylation levels of AKT, S6K, and 4EBP1, whereas the miR-486 inhibitor further reduced their phosphorylation levels. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001. NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: MiR-486 promotes AKT/mTOR signalling activation under OGD/R conditions in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of p-AKT/AKT expression in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative western blot images and quantitative analysis of p-S6K/S6K expression in H9c2 cells under the indicated conditions. ( C ) Representative western blot images and quantitative analysis of p-4EBP1/4EBP1 expression in H9c2 cells under the indicated conditions. Compared with OGD/R or mimic NC groups, miR-486 mimic significantly increased phosphorylation levels of AKT, S6K, and 4EBP1, whereas the miR-486 inhibitor further reduced their phosphorylation levels. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001. NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: p-AKT , Proteintech , 66444-1-1 g , 60–62.

Techniques: Activation Assay, Western Blot, Analysis, Expressing, Control, Phospho-proteomics, Negative Control

Primary antibodies used in this study

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: p-AKT , Proteintech , 66444-1-1 g , 60–62.

Techniques:

Antibodies used in this study

Journal: Journal of Animal Science and Biotechnology

Article Title: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways to promote myogenesis in pigs

doi: 10.1186/s40104-026-01504-w

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: p-AKT Ser473 , Proteintech , 66444-1-Ig.

Techniques: Cell-Signaling